4I9W

Human two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03318
Spacegroup name	P 1 21 1
Unit cell lengths	80.887, 138.905, 97.461
Unit cell angles	90.00, 94.87, 90.00

Refinement procedure

Resolution	48.600 - 2.750
R-factor	0.20264
Rwork	0.201
R-free	0.23564
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1ORS and 3UM7
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.600	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.077	0.031
Number of reflections	55209
<I/σ(I)>	20.1
Completeness [%]	99.6	97.3	95.8
Redundancy	4.2	4.1	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	50 mM Tris pH 8.5, 200 mM CaCl2, 27-30% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 277K