4I7D
Siah1 bound to synthetic peptide (ACE)KLRPVAMVRP(PRK)VR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 3 |
Unit cell lengths | 160.977, 160.977, 160.977 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.17296 |
Rwork | 0.171 |
R-free | 0.20167 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2A25 CHAIN A |
RMSD bond length | 0.005 |
RMSD bond angle | 1.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.073 | 0.662 |
Number of reflections | 27179 | |
<I/σ(I)> | 22.2 | 2.2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 65% MPD, 100 mM bicine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |