4I5B
Structure of human MHC class II protein HLA-DR1 carrying an influenza hemagglutinin peptide partially filling the binding groove
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-06-11 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.811, 111.381, 211.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.278 - 2.120 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.144 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 49.290 | 2.150 |
High resolution limit [Å] | 2.120 | 5.850 | 2.120 |
Number of reflections | 58717 | ||
Completeness [%] | 91.4 | 98 | 70 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 293 | 100 mM sodium acetate, 9% PEG 10000, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |