4I2X
Crystal structure of Signal Regulatory Protein gamma (SIRP-gamma) in complex with FabOX117
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 140.361, 174.191, 81.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.820 - 2.480 |
| R-factor | 0.19891 |
| Rwork | 0.196 |
| R-free | 0.25996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dif 2wng |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.157 | |
| Number of reflections | 71197 | |
| <I/σ(I)> | 14.9 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.3 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M magnesium acetate tetrahydrate, 20 % w/v polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
