4I0K
Crystal structure of murine B7-H3 extracellular domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9789 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 100.920, 100.920, 188.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.970 |
R-factor | 0.21712 |
Rwork | 0.215 |
R-free | 0.24974 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bis |
RMSD bond length | 0.012 |
RMSD bond angle | 1.742 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | BALBES |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 87.399 | 48.738 | 3.130 |
High resolution limit [Å] | 2.833 | 9.390 | 2.970 |
Rmerge | 0.041 | 0.800 | |
Number of reflections | 12250 | ||
<I/σ(I)> | 18.1 | 14.9 | 1 |
Completeness [%] | 99.7 | 97.5 | 100 |
Redundancy | 13.1 | 11.4 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 0.1 M HEPES, pH 7.5; 20% PEG-10000, VAPOR DIFFUSION, temperature 291K |
1 | VAPOR DIFFUSION | 7.5 | 291 | 0.1 M HEPES, pH 7.5; 20% PEG-10000, VAPOR DIFFUSION, temperature 291K |