4I0C

The structure of the camelid antibody cAbHuL5 in complex with human lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, Hamburg
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-13
Detector	MAR CCD 165 mm
Wavelength(s)	0.8125
Spacegroup name	P 43 21 2
Unit cell lengths	96.321, 96.321, 156.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.913 - 1.950
R-factor	0.1887
Rwork	0.187
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.014
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.913	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	54358
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4.8 M NaCl, 0.1 M HEPES pH 7, 3% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K