4HT1
Human TWEAK in complex with the Fab fragment of a neutralizing antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 3 |
| Unit cell lengths | 101.630, 101.630, 57.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.338 - 2.498 |
| R-factor | 0.1879 |
| Rwork | 0.183 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.625 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.338 |
| High resolution limit [Å] | 2.498 |
| Number of reflections | 22932 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% Ethanol, 10% PEG6000, 100 mM Sodium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
