4HNA
Kinesin motor domain in the ADP-MG-ALFX state in complex with tubulin and a DARPIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.050, 160.540, 174.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.140 - 3.190 |
R-factor | 0.1784 |
Rwork | 0.177 |
R-free | 0.21130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3RYC 2p4n 2xee |
RMSD bond length | 0.010 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.140 | 3.270 |
High resolution limit [Å] | 3.190 | 3.190 |
Number of reflections | 29641 | |
<I/σ(I)> | 19 | 4.56 |
Completeness [%] | 80.3 | 21.4 |
Redundancy | 25.8 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | PEG, PIPES BUFFER, PH 6.8, 0.8 MM ALCL3, 4 MM NAF, VAPOR DIFFUSION, HANGING DROP, temperature 293K |