4HLS
Crystal structure of mutant rabbit PRP 121-230 (S170N)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.520, 86.080, 86.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.510 - 1.450 |
| R-factor | 0.14733 |
| Rwork | 0.146 |
| R-free | 0.18017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o79 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.059 | 0.538 |
| Number of reflections | 39174 | |
| <I/σ(I)> | 24.29 | 3.6 |
| Completeness [%] | 97.3 | 73.2 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM SODIUM CACODYLATE PH 6.5, 3.0M NACL, 30% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
