4HKQ

XMRV reverse transcriptase in complex with RNA/DNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.872
Spacegroup name	P 43 21 2
Unit cell lengths	98.132, 98.132, 201.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.661 - 3.040
R-factor	0.227
Rwork	0.224
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1RW3
RMSD bond length	0.011
RMSD bond angle	1.011
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.090
High resolution limit [Å]	3.040	3.040
Number of reflections	19791
Completeness [%]	100.0	100
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	292	17% PEG 3350, 0.1M Bis-Tris, 0.2M Ammonium Sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	5	292	17% PEG 3350, 0.1M Bis-Tris, 0.2M Ammonium Sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K