4HKQ
XMRV reverse transcriptase in complex with RNA/DNA hybrid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.872 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.132, 98.132, 201.824 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.661 - 3.040 |
R-factor | 0.227 |
Rwork | 0.224 |
R-free | 0.27980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1RW3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.011 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.090 |
High resolution limit [Å] | 3.040 | 3.040 |
Number of reflections | 19791 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 17% PEG 3350, 0.1M Bis-Tris, 0.2M Ammonium Sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 17% PEG 3350, 0.1M Bis-Tris, 0.2M Ammonium Sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |