4HJ0
Crystal structure of the human GIPr ECD in complex with Gipg013 Fab at 3-A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.266, 109.850, 105.935 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 48.665 - 3.000 |
| R-factor | 0.2603 |
| Rwork | 0.255 |
| R-free | 0.31070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qkh 1gig |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (MR) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.665 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 21995 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 0.02 M TAPS, 30% (w/v) PEG 10,000, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
