4HFZ

Crystal Structure of an MDM2/P53 Peptide Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	296
Detector technology	PIXEL
Collection date	2011-02-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96
Spacegroup name	P 43 21 2
Unit cell lengths	52.944, 52.944, 196.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.120 - 2.694
R-factor	0.19777
Rwork	0.195
R-free	0.24679
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.634
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.680	2.850
High resolution limit [Å]	2.694	2.694
Number of reflections	8223
Completeness [%]	96.6	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	0.2 M (NH4)2SO4, pH 4.6 and 30% w/v PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K