4HD0
Mre11 ATLD17/18 mutation retains Tel1/ATM activity but blocks DNA double-strand break repair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1158 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.039, 70.189, 81.489 |
| Unit cell angles | 90.00, 109.31, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.300 |
| R-factor | 0.22546 |
| Rwork | 0.223 |
| R-free | 0.27653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.875 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 30428 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 288 | JCSG core 2 suite Qiagen cond number 7 ratio 1/1 protein solution-cryst solution, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
