4HBN
Crystal structure of the human HCN4 channel C-terminus carrying the S672R mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-12-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 95.467, 95.467, 115.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.25941 |
Rwork | 0.257 |
R-free | 0.29669 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.238 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 29.170 | 2.690 |
High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
Rmerge | 0.019 | 0.232 | |
Number of reflections | 8484 | ||
<I/σ(I)> | 65.4 | 7.6 | |
Completeness [%] | 99.1 | 93.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 277 | 1.6 M KH2PO4/Na2HPO4 and 0.1 M Phosphate-Citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |