4H7U
Crystal structure of pyranose dehydrogenase from Agaricus meleagris, wildtype
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-5 |
Synchrotron site | MAX II |
Beamline | I911-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.90772 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.938, 74.640, 139.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.700 - 1.600 |
R-factor | 0.17176 |
Rwork | 0.171 |
R-free | 0.20116 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jbv |
RMSD bond length | 0.016 |
RMSD bond angle | 1.853 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.700 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 72035 | |
<I/σ(I)> | 16.4 | 2.6 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 50 mM KH2PO4, 5% 2-methyl-2,4-pentanediol, 20% (w/v) polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |