4GYY
Crystal structure of human O-GlcNAc Transferase with UDP-5SGlcNAc and a peptide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 98.520, 136.480, 152.770 |
| Unit cell angles | 90.00, 103.51, 90.00 |
Refinement procedure
| Resolution | 59.946 - 1.850 |
| R-factor | 0.2187 |
| Rwork | 0.218 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.806 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.7.2_869) |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.950 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 166242 | |
| Completeness [%] | 99.7 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.6M Lithium Sulfate, 0.1M Bis Tris Propane pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
