4GYF

Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN, L-histidinol and phosphate

Replaces:  3UMU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	85.661, 86.679, 45.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.831 - 1.647
R-factor	0.1612
Rwork	0.160
R-free	0.18560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gc3
RMSD bond length	0.006
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.831
High resolution limit [Å]	1.647
Number of reflections	40890
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2.5 M SODIUM CHLORIDE, 0.1M IMIDAZOLE,0.5mM Zinc chloride, 100 mM histidinol phosphate , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K