4GYF
Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN, L-histidinol and phosphate
Replaces: 3UMUExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.661, 86.679, 45.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.831 - 1.647 |
R-factor | 0.1612 |
Rwork | 0.160 |
R-free | 0.18560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gc3 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.831 |
High resolution limit [Å] | 1.647 |
Number of reflections | 40890 |
Completeness [%] | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2.5 M SODIUM CHLORIDE, 0.1M IMIDAZOLE,0.5mM Zinc chloride, 100 mM histidinol phosphate , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |