4GUS

Crystal structure of LSD2-NPAC with H3 in space group P3221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00001
Spacegroup name	P 32 2 1
Unit cell lengths	101.083, 101.083, 177.372
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.036 - 2.230
R-factor	0.2087
Rwork	0.207
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gu1
RMSD bond length	0.009
RMSD bond angle	1.209
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.230
Number of reflections	51612
Completeness [%]	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	277	0.1M Ammonium Iodide, 0.1M MES, 10% PEG3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K