4GUQ
Structure of mutS139F p73 DNA binding domain complexed with 20BP DNA response element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.23 |
| Spacegroup name | P 61 |
| Unit cell lengths | 172.498, 172.498, 34.093 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 3.700 |
| R-factor | 0.30252 |
| Rwork | 0.302 |
| R-free | 0.31564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g82 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Number of reflections | 6475 | |
| <I/σ(I)> | 5.36 | 1.92 |
| Completeness [%] | 96.7 | 95.6 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris, 50-125 mM sodium acetate and 20-24% (w/v) PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
