4GTW
Crystal structure of mouse Enpp1 in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-14 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 31 |
| Unit cell lengths | 105.283, 105.283, 173.685 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.875 - 2.700 |
| R-factor | 0.2331 |
| Rwork | 0.231 |
| R-free | 0.27640 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.763 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.206 | 0.070 | 0.533 |
| Number of reflections | 58977 | ||
| <I/σ(I)> | 5.4 | ||
| Completeness [%] | 99.7 | 99.8 | 97.5 |
| Redundancy | 7.6 | 10.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | PEG 600, MgOAc, NaCl, ZnSO4, pH 4.5, vapor diffusion, temperature 293K |
