4GNF
Crystal Structure of NSD3 tandem PHD5-C5HCH domains complexed with H3 peptide 1-15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97838 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 60.030, 62.710, 62.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.700 - 1.550 |
| R-factor | 0.1625 |
| Rwork | 0.160 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gne |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.314 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.389 | 31.355 | 1.630 |
| High resolution limit [Å] | 1.550 | 4.900 | 1.550 |
| Rmerge | 0.042 | 0.423 | |
| Rmeas | 0.046 | 0.457 | |
| Rpim | 0.018 | 0.173 | |
| Total number of observations | 3528 | 17506 | |
| Number of reflections | 17540 | ||
| <I/σ(I)> | 13.2 | 28.9 | 3.4 |
| Completeness [%] | 99.8 | 93.4 | 100 |
| Redundancy | 6.8 | 6.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | 0.1M Hepes pH 7.5, 70% v/v MPD, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
