4GE6
Crystal structure of human protein tyrosine phosphatase PTPN9 (MEG2) complex with compound 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Spacegroup name | P 1 |
Unit cell lengths | 40.137, 57.774, 66.702 |
Unit cell angles | 77.22, 78.03, 80.01 |
Refinement procedure
Resolution | 50.000 - 1.400 |
Rwork | 0.198 |
R-free | 0.21520 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4ge5 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.201 |
Data reduction software | MOSFLM |
Data scaling software | HKL-3000 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.240 |
Rmerge | 0.096 |
Number of reflections | 134008 |
Completeness [%] | 87.2 |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 298 | 25% PEG3350, 0.2 M potassium thiocyanate, 10% ethylene glycol, 0.1 M Bis-Tris propane, pH 6.15, VAPOR DIFFUSION, HANGING DROP, temperature 298K |