4GDL
Crystal Structure of Human Atg12~Atg5 Conjugate in Complex with an N-terminal Fragment of Atg16L1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0092 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.601, 58.399, 91.802 |
Unit cell angles | 90.00, 130.04, 90.00 |
Refinement procedure
Resolution | 36.224 - 2.875 |
R-factor | 0.2186 |
Rwork | 0.215 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.510 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.875 | 6.240 | 2.900 |
Rmerge | 0.056 | 0.031 | 0.541 |
Number of reflections | 12548 | ||
<I/σ(I)> | 14.9 | ||
Completeness [%] | 98.6 | 98.9 | 92.4 |
Redundancy | 4.4 | 4.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PROTEIN: 10 MG/ML in 0.01M HEPES pH 7.0, 0.3 M NaCl, 0.001 M DTT; RESERVOIR: 0.1M BICINE pH9.0, 0.2 M sodium phosphate dibasic, 18% PEG 3350, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |