4G35
Mcl-1 in complex with a biphenyl cross-linked Noxa peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.040, 47.970, 68.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.310 - 2.000 |
| R-factor | 0.19389 |
| Rwork | 0.192 |
| R-free | 0.23879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.789 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.310 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.287 | |
| Number of reflections | 9801 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% Peg 8000, 225 mM CaCl2, 2% Ethylene Glycol,50 mM Epps (pH 8.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
