4G2C
DyP2 from Amycolatopsis sp. ATCC 39116
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.131, 77.952, 252.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.040 - 2.250 |
| R-factor | 0.1867 |
| Rwork | 0.184 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d3q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.040 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.088 | 0.556 |
| Number of reflections | 45753 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 98.9 | 95 |
| Redundancy | 4.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 100 mM sodium acetate, pH 5.0, 22 mM manganese chloride, 8% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
