4FWF
Complex structure of LSD2/AOF1/KDM1b with H3K4 mimic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2012-05-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.444, 98.544, 101.358 |
| Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.20705 |
| Rwork | 0.206 |
| R-free | 0.23694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fwe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.228 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.072 | 0.950 |
| Number of reflections | 30975 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 21% PEG3350, 200mM diammonium citrate, pH 7, VAPOR DIFFUSION, temperature 298K |
