4FQV

Crystal structure of broadly neutralizing antibody CR9114 bound to H7 influenza hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 42 21 2
Unit cell lengths	197.570, 197.570, 223.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.354 - 5.750
R-factor	0.3152
Rwork	0.314
R-free	0.33930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.209
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	5.820
High resolution limit [Å]	5.750	5.750
Number of reflections	13125
<I/σ(I)>	18	1.8
Completeness [%]	99.6	97.7
Redundancy	13.6	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	277	1 M lithium chloride, 10% PEG6000, and 100 mM MES, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K