4FL3
Structural and Biophysical Characterization of the Syk Activation Switch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-03 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.735, 71.034, 76.333 |
| Unit cell angles | 90.00, 103.93, 90.00 |
Refinement procedure
| Resolution | 46.230 - 1.900 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.230 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.097 | 0.539 |
| <I/σ(I)> | 2.72 | |
| Completeness [%] | 98.2 | 95.2 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 8% PEG 3350, 100 mM Hepes, 200 mM proline, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
