4FK5

Structure of the SAGA Ubp8(S144N)/Sgf11/Sus1/Sgf73 DUB module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	108
Detector technology	CCD
Collection date	2010-08-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	83.528, 104.775, 107.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.868 - 2.032
R-factor	0.17669
Rwork	0.175
R-free	0.21203
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.277
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.868	2.070
High resolution limit [Å]	2.032	2.032
Rmerge		0.865
Number of reflections	60890
<I/σ(I)>		4.64
Completeness [%]	99.5	100
Redundancy		14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	291	sodium citrate pH 5.2, 16% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K