4FIP
Structure of the SAGA Ubp8(S144N)/Sgf11(1-72, Delta-ZnF)/Sus1/Sgf73 DUB module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 108 |
Detector technology | CCD |
Collection date | 2010-11-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.973, 79.975, 274.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.180 - 2.686 |
R-factor | 0.18717 |
Rwork | 0.183 |
R-free | 0.26361 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mhs |
RMSD bond length | 0.012 |
RMSD bond angle | 1.545 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.686 | 2.686 |
Rmerge | 0.151 | 0.637 |
Number of reflections | 43495 | |
<I/σ(I)> | 12.227 | 2.345 |
Completeness [%] | 91.9 | 84.9 |
Redundancy | 7.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 14% PEG3350, 0.18-0.22 M Tri-ammonium citrate, 8-14% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |