4FIP

Structure of the SAGA Ubp8(S144N)/Sgf11(1-72, Delta-ZnF)/Sus1/Sgf73 DUB module

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Ubiquitin carboxyl-terminal hydrolase 8	polymer	476	54060.7	2	UniProt (P50102) Pfam (PF02148) Pfam (PF00443) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Deubiquitinating enzyme 8, Ubiquitin thioesterase 8, Ubiquitin-specific-processing protease 8
2	B, F	Protein SUS1	polymer	96	11094.5	2	UniProt (Q6WNK7) Pfam (PF10163) In PDB	Saccharomyces cerevisiae (Baker's yeast)
3	C, G	SAGA-associated factor 11	polymer	72	8295.2	2	UniProt (Q03067) Pfam (PF18519) In PDB	Saccharomyces cerevisiae (Baker's yeast)	11 kDa SAGA-associated factor
4	D, H	SAGA-associated factor 73	polymer	96	10824.3	2	UniProt (P53165) Pfam (PF18508) In PDB	Saccharomyces cerevisiae (Baker's yeast)	73 kDa SAGA-associated factor, SAGA histone acetyltransferase complex 73 kDa subunit
5	A, D, E, H	ZINC ION	non-polymer		65.4	14	Chemie (ZN)
6		water	water		18.0	92	Chemie (HOH)

Sequence modifications

A, E: 1 - 471 (UniProt: P50102)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ala 0	-	expression tag
Asn 144	Ser 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	168549.2
Non-Polymers*	Number of molecules	14
	Total formula weight	915.7
All*	Total formula weight	169464.9

*Water molecules are not included.