4FIC
Kinase domain of cSrc in complex with a hinge region-binding fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2012-02-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979400 |
Spacegroup name | P 1 |
Unit cell lengths | 42.034, 63.013, 73.561 |
Unit cell angles | 79.63, 88.12, 90.17 |
Refinement procedure
Resolution | 43.360 - 2.500 |
R-factor | 0.1997 |
Rwork | 0.198 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oiq |
RMSD bond length | 0.013 |
RMSD bond angle | 1.473 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.600 | |
High resolution limit [Å] | 2.500 | 16.000 | 2.500 |
Rmerge | 0.066 | 0.031 | 0.347 |
Number of reflections | 25142 | 94 | 2800 |
<I/σ(I)> | 14.18 | 37.97 | 4.06 |
Completeness [%] | 97.8 | 94 | 97.1 |
Redundancy | 3.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 11% ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |