4FBN
Insights into structural integration of the PLCgamma regulatory region and mechanism of autoinhibition and activation based on key roles of SH2 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9163 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 53.752, 59.610, 76.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.060 - 2.400 |
| R-factor | 0.2224 |
| Rwork | 0.219 |
| R-free | 0.29540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.060 | 2.640 |
| High resolution limit [Å] | 2.400 | 2.500 |
| Rmerge | 0.695 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 96.2 | 96.2 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 22% PEG 5K MME, 0.1M Na Malonate 20mM CaCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
