4FBN
Insights into structural integration of the PLCgamma regulatory region and mechanism of autoinhibition and activation based on key roles of SH2 domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-31 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9163 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 53.752, 59.610, 76.923 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.060 - 2.400 |
R-factor | 0.2224 |
Rwork | 0.219 |
R-free | 0.29540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.060 | 2.640 |
High resolution limit [Å] | 2.400 | 2.500 |
Rmerge | 0.695 | |
<I/σ(I)> | 2.5 | |
Completeness [%] | 96.2 | 96.2 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 22% PEG 5K MME, 0.1M Na Malonate 20mM CaCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |