4FAT
Ligand-binding domain of GluA2 (flip) ionotropic glutamate receptor in complex with an allosteric modulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95375 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.866, 88.143, 47.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.400 |
| R-factor | 0.17016 |
| Rwork | 0.168 |
| R-free | 0.20938 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o28 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.291 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.052 | 0.162 |
| Number of reflections | 48434 | |
| <I/σ(I)> | 11.4 | 4.1 |
| Completeness [%] | 91.0 | 61.9 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 298 | 18% PEG 4000, 50mM Lithium Sulphate, 100mM Sodium Cacodylate, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
