4F3L

Crystal Structure of the Heterodimeric CLOCK:BMAL1 Transcriptional Activator Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2011-06-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	67.242, 71.955, 173.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.117 - 2.268
R-factor	0.186
Rwork	0.184
R-free	0.21710
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.889
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.268
Number of reflections	39307
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	100 mM Hepes pH 8.0, 6% PEG 3350 and 75 mM NaF., VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	8	293	100 mM Hepes pH 8.0, 6% PEG 3350 and 75 mM NaF., VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	8	293	100 mM Hepes pH 8.0, 6% PEG 3350 and 75 mM NaF., VAPOR DIFFUSION, temperature 293K