4F3F

Crystal Structure of Msln7-64 MORAb-009 FAB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 64
Unit cell lengths	146.163, 146.163, 80.874
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.076 - 2.650
R-factor	0.1794
Rwork	0.177
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f33 Difference Fourier revealed MSLN7-64
RMSD bond length	0.017
RMSD bond angle	1.584
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: dev_965))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.610	2.610
Number of reflections	29264
<I/σ(I)>		1.76
Completeness [%]	97.8	80
Redundancy		2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294	0.1 M Trisodium citrate pH 5.6, 17% Polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K