4F2C

The Crystal Structure of a Human MitoNEET double mutant in which Gly 66 are Asp 67 are both Replaced with Ala Residues

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	45.632, 51.995, 59.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.014 - 1.350
R-factor	0.154
Rwork	0.153
R-free	0.18120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qh7
RMSD bond length	0.008
RMSD bond angle	1.525
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.014	59.018	1.390
High resolution limit [Å]	1.350	6.040	1.350
Rmerge		0.033	0.657
Total number of observations		3782	8899
Number of reflections	31045
<I/σ(I)>	16.8	15.8	1.1
Completeness [%]	98.3	99.8	85.1
Redundancy	9.2	8.9	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1