4F1H

Crystal structure of TDP2 from Danio rerio complexed with a single strand DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	96
Detector technology	CCD
Collection date	2011-12-09
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	57.918, 95.100, 104.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.871 - 1.662
R-factor	0.1644
Rwork	0.164
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.528
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.660
Number of reflections	67804
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	293	PEG and sodium tartrate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K