4EW3
The structure of human glycinamide ribonucleotide transformylase in complex with 10R-methylthio-DDATHF.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.124, 78.124, 230.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.730 - 1.702 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1meo |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.730 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 46588 | |
| <I/σ(I)> | 38.3 | 38.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 0.1 M phosphate/citrate buffer, 1.5-2.0 M ammonium sulfate at p.H 4.2. 25% Glycerol added as , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
