4EUW
Crystal structure of a HMG domain of transcription factor SOX-9 bound to DNA (SOX-9/DNA) from Homo sapiens at 2.77 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-21 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 55.772, 134.654, 49.987 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.920 - 2.770 |
R-factor | 0.24826 |
Rwork | 0.247 |
R-free | 0.27835 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f27 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.399 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC (5.7.0127) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.922 | 27.920 | 2.840 |
High resolution limit [Å] | 2.770 | 12.390 | 2.770 |
Rmerge | 0.066 | 0.016 | 0.575 |
Total number of observations | 18522 | ||
Number of reflections | 4880 | 61 | 351 |
<I/σ(I)> | 15.3 | 38.4 | 2.6 |
Completeness [%] | 96.6 | 87.8 | 98.8 |
Redundancy | 3.8 | 3.08 | 3.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 30.00% polyethylene glycol 8000, 0.20M ammonium acetate, 0.010 M magnesium acetate, 0.1M sodium cacodylate pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |