4EUP
The complex between TCR JKF6 and human Class I MHC HLA-A2 presenting the MART-1(27-35)(A27L) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 127.655, 53.700, 150.514 |
| Unit cell angles | 90.00, 112.46, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.880 |
| R-factor | 0.23806 |
| Rwork | 0.235 |
| R-free | 0.29488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4EUR AND 2GTZ |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.582 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.960 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 37988 | |
| <I/σ(I)> | 15.9 | 2.5 |
| Completeness [%] | 93.8 | 95 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Bis-Tris, 16% PEG10000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
