4EJ8
Apo HIV Protease (PR) dimer in closed form with fragment 1F1 in the outside/top of flap
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-04 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.947, 66.533, 91.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.850 - 2.347 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.68A) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.854 | 2.450 |
| High resolution limit [Å] | 2.347 | 2.347 |
| Number of reflections | 7885 | |
| <I/σ(I)> | 8.14 | 1.93 |
| Completeness [%] | 99.4 | 95.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1 M Tris, pH 7.5, 28% PEG4000, 10% DMSO, VAPOR DIFFUSION, temperature 298K |
