4ED5
Crystal structure of the two N-terminal RRM domains of HuR complexed with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.770, 62.750, 53.290 |
| Unit cell angles | 90.00, 111.89, 90.00 |
Refinement procedure
| Resolution | 35.080 - 2.000 |
| R-factor | 0.21115 |
| Rwork | 0.209 |
| R-free | 0.25651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.080 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.190 | |
| Number of reflections | 27777 | |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 98.3 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 18% PEG 5000, 0.1M HEPES, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
