4E7K
PFV integrase Target Capture Complex (TCC-Mn), freeze-trapped prior to strand transfer, at 3.0 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 163.914, 163.914, 129.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.009 - 3.020 |
R-factor | 0.2469 |
Rwork | 0.245 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3os2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.313 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.180 |
High resolution limit [Å] | 3.020 | 3.020 |
Rmerge | 0.109 | 0.449 |
Number of reflections | 35032 | |
<I/σ(I)> | 6.3 | 2 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.4 | 291 | 34% PEG-400, 180MM LI2SO4,2.5MM EDTA, 100MM TRIS-HCL, pH 7.4, VAPOR DIFFUSION, temperature 291K |