4E45
Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi Anemia Associated Helicase hFANCM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033180 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.980, 69.970, 116.050 |
Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
Resolution | 49.510 - 2.000 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1taf |
RMSD bond length | 0.015 |
RMSD bond angle | 1.520 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.510 | 2.050 | |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.052 | 0.033 | 0.512 |
Number of reflections | 119106 | 872 | 8864 |
<I/σ(I)> | 14.9 | 29.3 | 3 |
Completeness [%] | 98.9 | 60.3 | 99.7 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 298 | Tracking Code C4, 0.1M Tris pH 7.8, 0.2M LiCl, 0.1M Na2SO4, 17.5% w/v PEG3350, 10% glycerol cryo., vapor diffusion, sitting drop, temperature 298K |