Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97610 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.290, 86.259, 46.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.300 - 1.540 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.124 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.297 | 48.297 | 1.620 |
| High resolution limit [Å] | 1.540 | 4.870 | 1.540 |
| Rmerge | 0.063 | 0.023 | 0.791 |
| Total number of observations | 4485 | 7120 | |
| Number of reflections | 29415 | ||
| <I/σ(I)> | 14.7 | 26.4 | 1 |
| Completeness [%] | 83.7 | 98.2 | 42 |
| Redundancy | 3.9 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 1.0 M KSCN, 0.1 M BIS-TRIS-HCL, 10% DMSO, pH 7.0, VAPOR DIFFUSION, temperature 277K |
