4E41
Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor G4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.133, 175.611, 88.646 |
Unit cell angles | 90.00, 110.75, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.20076 |
Rwork | 0.197 |
R-free | 0.26374 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iam |
RMSD bond length | 0.011 |
RMSD bond angle | 1.687 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.269 | |
Number of reflections | 53716 | |
<I/σ(I)> | 2.6 | |
Completeness [%] | 97.2 | 83.8 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 298 | 20% (wt/vol) polyethylene glycol 1000, 0.2 M calcium acetate, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, temperature 298K |