4E3X
Crystal Structure of Mus musculus 1-pyrroline-5-carboxylate dehydrogenase cryoprotected in proline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.980, 93.878, 132.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.479 - 1.240 |
R-factor | 0.1562 |
Rwork | 0.155 |
R-free | 0.18030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v9j |
RMSD bond length | 0.005 |
RMSD bond angle | 1.078 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.639 | 45.639 | 1.310 |
High resolution limit [Å] | 1.240 | 3.920 | 1.240 |
Rmerge | 0.032 | 0.550 | |
Total number of observations | 44859 | 123860 | |
Number of reflections | 279378 | ||
<I/σ(I)> | 15 | 15.7 | 1.4 |
Completeness [%] | 94.1 | 97.6 | 87.3 |
Redundancy | 4.1 | 4.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 294 | 20% PEG 3350, 0.1 M Bis-Tris, pH 6.25, VAPOR DIFFUSION, SITTING DROP, temperature 294K |