4E36

Crystal structure of the human Endoplasmic Reticulum Aminopeptidase 2 variant N392K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	74.340, 134.450, 127.370
Unit cell angles	90.00, 90.85, 90.00

Refinement procedure

Resolution	10.996 - 3.220
R-factor	0.2113
Rwork	0.209
R-free	0.26080
Structure solution method	isomorphous replacement
Starting model (for MR)	3se6
RMSD bond length	0.007
RMSD bond angle	1.120
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.450	3.390
High resolution limit [Å]	3.220	3.220
Number of reflections	71589
Completeness [%]	99.1	99.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	10% PEG 8000, 20% ethylene glycol, 20 mM glycine, 20 mM DL-lysine, 20 mM DL-serine, 20 mM DL-alanine, 20 mM sodium L-glutamate, 31 mM imidazole, 69 mM MES. Protein mixed 1:4 with LPL peptide, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K