4E18

Alpha-E-catenin is an autoinhibited molecule that co-activates vinculin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	193
Detector technology	CCD
Collection date	2011-11-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	52.233, 68.102, 99.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.787 - 2.403
R-factor	0.2217
Rwork	0.218
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t01
RMSD bond length	0.003
RMSD bond angle	0.657
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.063	0.027	0.319
Number of reflections	14046
<I/σ(I)>	10
Completeness [%]	97.4	94.6	87.5
Redundancy	3.5	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	22% PEG3350, 100 mM bis-tris-propane, pH 8.0, 100 mM sodium nitrate, 3 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K